A bi-stable display, frame updating method and timing sequence controlling method thereof are disclosed. The frame updating method of the bi-stable display includes the steps of comparing a difference of source driver output voltages of adjacent frames of image updating period with a preset threshold, and determining parameter value of each transitional state of the adjacent frames when the difference equal or greater than the preset threshold for controlling level of source driver output voltage. Accordingly, the effects such as signal interference, reduction of image quality resulted from switching between high voltage levels directly and rapidly and excessive power consumption may be eliminated.