Invention Grant
- Patent Title: Solid-phase detection of terminal monosaccharides cleaved from glycosylated substrates
- Patent Title (中): 从糖基底物切割的末端单糖的固相检测
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Application No.: US12373331Application Date: 2007-07-10
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Publication No.: US08445288B2Publication Date: 2013-05-21
- Inventor: Mads Detlef Sorensen , Rita Martins , Ole Hindsgaul
- Applicant: Mads Detlef Sorensen , Rita Martins , Ole Hindsgaul
- Applicant Address: DE Darmstadt
- Assignee: Merck Patent GmbH
- Current Assignee: Merck Patent GmbH
- Current Assignee Address: DE Darmstadt
- Agency: Millen, White, Zelano & Branigan, P.C.
- Priority: DK200600969 20060712
- International Application: PCT/DK2007/000352 WO 20070710
- International Announcement: WO2008/006373 WO 20080117
- Main IPC: G01N33/00
- IPC: G01N33/00 ; C12Q1/54
Abstract:
The present invention relates to a novel method for analysing carbohydrates. The invention is in particular useful in detecting a terminal monosaccharide which may be released from a glycosylated substrate for example using an exoglucosidase. After relase from the glycosylated substrate the terminal monosaccharide may be captured on a solid support, incubated with a boronate detection agent and detected by aid of the boronate detection agent. The methods of the invention are useful for a variety of purposes including sequencing of carbohydrates, wherein exoglycosidases with predetermined specificity are employed for the release.
Public/Granted literature
- US20100047828A1 SOLID-PHASE DETECTION OF TERMINAL MONOSACCHARIDES CLEAVED FROM GLYCOSYLATED SUBSTRATES Public/Granted day:2010-02-25
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