An over-current protection device for multiple high-voltage motive devices is provided. The over-current protection device includes a comparison module and a logic operation module. The comparison module receives a plurality of voltages generated from a plurality of operating currents of a plurality of high-voltage motive devices and respectively compares the voltages with at least one reference voltage to generate a plurality of comparison results, wherein the high-voltage motive devices are solenoids, electronic clutches, or a combination of solenoids and electronic clutches. The logic operation module receives the comparison results and generates at least one control signal for a plurality of high-voltage motive device driving circuits according to the comparison results. The high-voltage motive device driving circuits respectively drive the high-voltage motive devices according to the control signal.