Circuits and methods are provided for estimating a latency through a FIFO buffer. A first detector detects first instances of a pattern in first data values serially written to a write port of the FIFO buffer. A second detector detects second instances of the pattern in second data values serially read from a read port of the FIFO buffer. The second data values are the first data values delayed by the latency through the FIFO buffer. A counter counts a count of active transitions of a sample clock signal. The counter starts on each detected first instance and stops on each detected second instances. The count provides an estimate of the latency of the FIFO buffer.