Invention Grant
- Patent Title: Detection and quantification of biomolecules using mass spectrometry
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Application No.: US13040056Application Date: 2011-03-03
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Publication No.: US08383795B2Publication Date: 2013-02-26
- Inventor: Dirk Johannes Van Den Boom , Paul Andrew Oeth , Payam Mahboubi
- Applicant: Dirk Johannes Van Den Boom , Paul Andrew Oeth , Payam Mahboubi
- Applicant Address: US CA San Diego
- Assignee: Sequenom, Inc.
- Current Assignee: Sequenom, Inc.
- Current Assignee Address: US CA San Diego
- Agency: Grant Anderson LLP
- Main IPC: C07H21/02
- IPC: C07H21/02 ; C07H21/04 ; C07H19/04 ; C12Q1/68 ; C12P19/34
Abstract:
The present invention is directed in part to a method for detecting a target nucleic acid using detector oligonucleotides detectable by mass spectrometry. This method takes advantage of the 5′ to 3′ nuclease activity of a nucleic acid polymerase to cleave annealed oligonucleotide probes from hybridized duplexes and releases labels for detection by mass spectrometry. This process is easily incorporated into a polymerase chain reaction (PCR) amplification assay. The method also includes embodiments directed to quantitative analysis of target nucleic acids.
Public/Granted literature
- US20110160093A1 DETECTION AND QUANTIFICATION OF BIOMOLECULES USING MASS SPECTROMETRY Public/Granted day:2011-06-30
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