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US08257758B2 Process for the extraction, purification and enzymatic modification of soy 7S globulin alpha' subunit for use as hypocholesterolemizing agent 有权
大豆7S球蛋白α'亚基的提取，纯化和酶修饰方法，用作降血胆固醇剂

  • Patent Title: Process for the extraction, purification and enzymatic modification of soy 7S globulin alpha' subunit for use as hypocholesterolemizing agent
  • Patent Title (中): 大豆7S球蛋白α'亚基的提取，纯化和酶修饰方法，用作降血胆固醇剂
  • Application No.: US13013966
    Application Date: 2011-01-26
  • Publication No.: US08257758B2
    Publication Date: 2012-09-04
  • Inventor: Marcello Duranti , Paolo Morazzoni
  • Applicant: Marcello Duranti , Paolo Morazzoni
  • Applicant Address: IT Milan
  • Assignee: Indena S.p.A.
  • Current Assignee: Indena S.p.A.
  • Current Assignee Address: IT Milan
  • Agency: Young & Thompson
  • Priority: ITMI2002A0147 20020129
  • Main IPC: A61K36/48
  • IPC: A61K36/48
Process for the extraction, purification and enzymatic modification of soy 7S globulin alpha' subunit for use as hypocholesterolemizing agent
Abstract:
A purified amino-terminal polypeptide fragment of the soy β-conglycinin α′ subunit is prepared by selective extraction of defatted soy with an aqueous solution of sodium bisulfite, precipitation with ethanol, and Metal Affinity Chromatography (MAC) under denaturing conditions to obtain the α′ subunit. The α′ subunit is then enzymatically treated with chymotrypsin and subjected to further MAC to recover the amino-terminal fragment of the polypeptide (MW 28,000 Da).
Abstract(Chinese):

通过在变性条件下用亚硫酸氢钠水溶液，用乙醇沉淀和金属亲和层析（MAC）选​​择性提取脱脂大豆来制备大豆和大豆球蛋白α'亚基的纯化的氨基末端多肽片段，得到 α'亚基。 然后用胰凝乳蛋白酶对α'亚基进行酶处理，并进一步进行MAC以回收多肽的氨基末端片段（MW 28,000Da）。

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