A bio-air sterilization system and method of use thereof is provided that can remove and render benign harmful contaminants and particulates, such as bacteria, viruses, and molds, from air within an enclosed area, as well as, in principle, from the exposed surfaces located within the enclosed area. In one aspect, the sterilization system includes a self contained, mobile sterilization unit that includes at least an ultraviolet array, an air flow control mechanism for diverting the air flow within the system through either a filter or through an ozone removal zone, an ozone generator, and a blower apparatus to pull the air through the system and out through ports, such as a nozzle system, to the surrounding environment.