The combination of Nα-long chain alkanoyl di basic amino acid alkyl ester salts with glycerol monofatty acid esters results in biocidal synergy and the extension of cidal activity of the di basic amino acid derivative for a variety of antimicrobial applications like oral care, wound care, dermatological care, animal care, and cosmetic applications.Nα-C8-C14-long chain alkanoyl-L-arginine alkyl (C1-C4 short chain) ester salts with glycerol monofatty acid esters (C8-C14) are very effective as antimicrobial agents for hospital use particularly as a coating for surgical drapes, covers, walls, trays, table tops, gurneys, or the like. Other uses include the treatment of HSV-1 (fever sore) and as a microbicide when using the combination of the di basic amino acid ester derivative with glycerol monofatty acid esters for preventing HIV and STD's.