Invention Grant
US08192990B2 Buffer solution for electroporation and a method comprising the use of the same
有权
用于电穿孔的缓冲溶液和包括其使用的方法
- Patent Title: Buffer solution for electroporation and a method comprising the use of the same
- Patent Title (中): 用于电穿孔的缓冲溶液和包括其使用的方法
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Application No.: US13009024Application Date: 2011-01-19
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Publication No.: US08192990B2Publication Date: 2012-06-05
- Inventor: Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Mueller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterhus , Michael Nix
- Applicant: Gudula Riemen , Elke Lorbach , Juliana Helfrich , Gregor Siebenkotten , Herbert Mueller-Hartmann , Kirsten Rothmann-Cosic , Corinna Thiel , Meike Weigel , Heike Wessendorf , Helmut Brosterhus , Michael Nix
- Applicant Address: DE Cologne
- Assignee: Lonza Cologne GmbH
- Current Assignee: Lonza Cologne GmbH
- Current Assignee Address: DE Cologne
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: DE10120000 20010423
- Main IPC: C12N15/87
- IPC: C12N15/87 ; C12N13/00

Abstract:
A method for introducing biologically active molecules into animal or human cells using an electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution including HEPES and at least 10 mmol×1−1 magnesium ions (Mg2+), the buffer solution having a buffer capacity of at least 20 mmol×1−1 ×pH−1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C., and an ionic strength of at least 200 mmol×1−1. An electric voltage is applied to the suspension.
Public/Granted literature
- US20110117654A1 BUFFER SOLUTION FOR ELECTROPORATION AND A METHOD COMPRISING THE USE OF THE SAME Public/Granted day:2011-05-19
Information query
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