Invention Grant
US08192990B2 Buffer solution for electroporation and a method comprising the use of the same 有权
用于电穿孔的缓冲溶液和包括其使用的方法

Buffer solution for electroporation and a method comprising the use of the same
Abstract:
A method for introducing biologically active molecules into animal or human cells using an electric current includes suspending the cells and dissolving the biologically active molecules in a buffer solution including HEPES and at least 10 mmol×1−1 magnesium ions (Mg2+), the buffer solution having a buffer capacity of at least 20 mmol×1−1 ×pH−1 at a change in the pH from pH 7 to pH 8 and at a temperature of 25° C., and an ionic strength of at least 200 mmol×1−1. An electric voltage is applied to the suspension.
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