Invention Grant
US08192932B2 Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase
有权
将DNA链与牛痘拓扑异构酶催化的RNA链共价连接
- Patent Title: Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase
- Patent Title (中): 将DNA链与牛痘拓扑异构酶催化的RNA链共价连接
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Application No.: US12658764Application Date: 2010-02-12
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Publication No.: US08192932B2Publication Date: 2012-06-05
- Inventor: Stewart Shuman , JoAnn Sekiguchi , John Comiskey , Joseph Fernandez , James Hoeffler , Robert Marcil
- Applicant: Stewart Shuman , JoAnn Sekiguchi , John Comiskey , Joseph Fernandez , James Hoeffler , Robert Marcil
- Applicant Address: US NY New York US CA Carlsbad
- Assignee: Sloan-Kettering Institute For Cancer Research,Invitrogen Corporation
- Current Assignee: Sloan-Kettering Institute For Cancer Research,Invitrogen Corporation
- Current Assignee Address: US NY New York US CA Carlsbad
- Agency: Woodcock Washburn, LLP
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P21/06 ; C12P19/34 ; C07H21/02
Abstract:
The present invention provides a method of covalently joining a DNA strand to an RNA strand, a method of tagging a 5′ end of an RNA molecule, a DNA-RNA molecule which has been joined in vitro by the use of a topoisomerase, a method of tagging a 5′ end of an mRNA, and a method of isolating and cloning full-length gene sequences using capped mRNA after subtraction of non-capped RNA.
Public/Granted literature
- US20100317064A1 Covalent joining of DNA strands to RNA strands catalyzed by vaccinia topoisomerase Public/Granted day:2010-12-16
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