Invention Grant
US08168856B2 Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of adpglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of adpglucose and starch
有权
生产重组蔗糖合成酶的方法,其用于制备用于测定蔗糖,葡萄糖生产和转基因植物的生产的试剂盒,其叶和储存器官积累高含量的葡萄糖和淀粉
- Patent Title: Method of production of recombinant sucrose synthase, use thereof in the manufacture of kits for determination of sucrose, production of adpglucose and production of transgenic plants whose leaves and storage organs accumulate high contents of adpglucose and starch
- Patent Title (中): 生产重组蔗糖合成酶的方法,其用于制备用于测定蔗糖,葡萄糖生产和转基因植物的生产的试剂盒,其叶和储存器官积累高含量的葡萄糖和淀粉
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Application No.: US10587372Application Date: 2005-01-27
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Publication No.: US08168856B2Publication Date: 2012-05-01
- Inventor: Miren Edurne Baroja Fernandez , Francisco José Muñoz Perez , Francisco Javier Pozueta Romero , Maria Teresa Moran Zorzano , Nora Alonso Casajus
- Applicant: Miren Edurne Baroja Fernandez , Francisco José Muñoz Perez , Francisco Javier Pozueta Romero , Maria Teresa Moran Zorzano , Nora Alonso Casajus
- Applicant Address: ES Pamplona, Navarra ES Madrid
- Assignee: Universidad Publica de Navarra,Consejo Superior de Investigaciones Cientificas
- Current Assignee: Universidad Publica de Navarra,Consejo Superior de Investigaciones Cientificas
- Current Assignee Address: ES Pamplona, Navarra ES Madrid
- Agency: Ladas & Parry LLP
- Priority: ES200400257 20040205
- International Application: PCT/ES2005/070010 WO 20050127
- International Announcement: WO2005/075649 WO 20050818
- Main IPC: A01H5/00
- IPC: A01H5/00 ; C12N15/82 ; C12N15/00 ; C12N15/10

Abstract:
An isolated sucrose synthase peptide. Also, a method of preparing ADPglucose by incubating the isolated sucrose synthase peptide with ADP in suitable conditions and then isolating and purifying the ADPG produced. Also, an assay kit for the spectrophotometric, fluorimetric or amperometric determination of sucrose, which kit includes the isolated sucrose synthase peptide. Also, a method of producing a transgenic plant that overexpresses sucrose synthase by inserting a genetic construct containing a DNA fragment that encodes the sucrose synthase peptide into a vector and transferring to a plant genome, and a transgenic plant obtained thereby.
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