Invention Grant
- Patent Title: Highly thermostable fluorescent proteins
- Patent Title (中): 高度耐热的荧光蛋白
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Application No.: US13306735Application Date: 2011-11-29
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Publication No.: US08168411B2Publication Date: 2012-05-01
- Inventor: Andrew M. Bradbury , Geoffrey S. Waldo , Csaba Kiss
- Applicant: Andrew M. Bradbury , Geoffrey S. Waldo , Csaba Kiss
- Applicant Address: US NM Los Alamos
- Assignee: Los Alamos National Security, LLC
- Current Assignee: Los Alamos National Security, LLC
- Current Assignee Address: US NM Los Alamos
- Agent Bruce H. Cottrell; Kenneth K. Sharples; Meredith H. Schoenfeld
- Main IPC: C12P21/06
- IPC: C12P21/06 ; C12N1/20 ; C12N15/00 ; C07H21/02
Abstract:
Thermostable fluorescent proteins (TSFPs), methods for generating these and other stability-enhanced proteins, polynucleotides encoding such proteins, and assays and method for using the TSFPs and TSFP-encoding nucleic acid molecules are provided. The TSFPs of the invention show extremely enhanced levels of stability and thermotolerance. In one case, for example, a TSFP of the invention is so stable it can be heated to 99° C. for short periods of time without denaturing, and retains 85% of its fluorescence when heated to 80° C. for several minutes. The invention also provides a method for generating stability-enhanced variants of a protein, including but not limited to fluorescent proteins.
Public/Granted literature
- US20120077266A1 HIGHLY THERMOSTABLE FLUORESCENT PROTEINS Public/Granted day:2012-03-29
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