The present invention discloses an integrated circuit for system calibration, applicable to a power supply, comprising: a comparison module, having a feedback input end coupled to a feedback signal and a reference input end coupled to an analog reference signal for delivering a status signal; a detection and control module, for generating a reference signal and a calibration value according to the status signal, wherein the calibration value is derived from the reference signal at an instant when the status signal changes state, and the calibration value is stored into a calibration value register; a memory module, for receiving, storing and outputting the calibration value; and a reference signal generator, receiving the calibration value to provide the analog reference signal. The present invention can therefore be used to automatically calibrate a system with fewer external components to provide qualified systems.