Invention Grant
- Patent Title: Massive parallel method for decoding DNA and RNA
- Patent Title (中): 用于解码DNA和RNA的大规模并行方法
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Application No.: US12804284Application Date: 2010-07-19
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Publication No.: US08088575B2Publication Date: 2012-01-03
- Inventor: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
- Applicant: Jingyue Ju , Zengmin Li , John Robert Edwards , Yasuhiro Itagaki
- Applicant Address: US NY New York
- Assignee: The Trustees of Columbia University in the City of New York
- Current Assignee: The Trustees of Columbia University in the City of New York
- Current Assignee Address: US NY New York
- Agency: Cooper & Dunham LLP
- Agent John P. White
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C07H21/00 ; C07H21/02

Abstract:
This invention provides methods for attaching a nucleic acid to a solid surface and for sequencing nucleic acid by detecting the identity of each nucleotide analogue after the nucleotide analogue is incorporated into a growing strand of DNA in a polymerase reaction. The invention also provides nucleotide analogues which comprise unique labels attached to the nucleotide analogue through a cleavable linker, and a cleavable chemical group to cap the —OH group at the 3′-position of the deoxyribose.
Public/Granted literature
- US20100317012A1 Massive parallel method for decoding DNA and RNA Public/Granted day:2010-12-16
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