A particle separation apparatus includes a base, a disc-shaped container rotatable on the base at a constant speed and a suspension supply tank for separating in the container particles contained in a suspension supplied from the tank according to specific gravities or particle diameters of the particles. The container includes a plurality of centrifugation vessels disposed around a rotation axis and in a circumferential direction of the container, particle supply cylinders disposed at a center of the container for discharging the particles in the suspension toward the vessels and a lid, and has a structure controllable in rotational speed to adjust a distance of relative movement in a rotation direction between large-gravity particles and small-gravity particles. The vessels are individually in a form of dents independent of one another and are each formed of an inner circumferential wall and a bottom wall. Each of the cylinders has means for discharging the particles in the suspension toward a centrifugation vessel filled beforehand with water.