A method of adjusting gain balance of an optical transducer includes the following steps. First, two external gains are predetermined. Next, the projection position of a reflection spot is adjusted toward one side of the optical transducer, and the current output values of the optical transducer each corresponding to one of the external gains are measured, respectively. Next, the projection position of the reflection spot is adjusted toward the other side of the optical transducer, and the current output values of the optical transducer each corresponding to one of the external gains are measured, respectively. Then, a gain balance value of the optical transducer is adjusted, and is set as the external gain. Next, the projection position of the reflection spot is adjusted. Finally, the projection position of the reflection spot, where the current output value of the transducer is equal to 0, is locked to complete the adjustment rapidly.