An image acquiring apparatus for acquiring images of a sample includes a macro image acquiring unit 20 for acquiring a macro image of the sample, a dark field light source 26 to be used for acquiring a dark field macro image of the sample as a macro image, a macro image processing unit 66 which generates a reference macro image by processing image data of the macro image, and an image pickup condition setting unit 65 which sets an image acquiring range corresponding to a range including an object of image acquisition as an image pickup condition of a micro image of the sample by referring to the reference macro image. This realizes an image acquiring apparatus, an image acquiring method, and an image acquiring program by which a macro image of a sample as an object of image acquisition is preferably acquired.