To present an automatic cell cultivation apparatus capable of sterilizing completely including a cold storage unit and a normal temperature storage unit, without causing cross contamination, in used of cell cultivation of plural subjects. An automatic cell cultivation apparatus comprising an operation unit 11 for operating to cultivate cells of subjects, incubator units 14 for cultivating the cells, a cold storage unit 16 for storing reagents necessary for cultivation, a normal temperature storage unit 17 for storing culture tools, an inlet-outlet unit 15 for taking in and out reagents and culture tools, and a steam supply unit 22 for autoclave sterilization, in which the incubator units 14, the cold storage unit 16, the normal temperature storage unit 17, and the inlet-outlet unit 15 communicate with the operation unit 11, and have sealing doors 18a to 18d leading to the operation unit 11, filters 20a and 20b for cleaning and sterilizing the air stream supplied in the operation unit 11 are provided, and sealing doors 30a and 30b are provided between the filter units and the operation unit 11. By selecting the sealing doors 18a to 18d and opening and closing, sterilizing steam from the steam supply unit 22 is supplied into any of the sterilization required units, operation unit 11, and the incubator units 14, the cold storage unit 16, the normal temperature storage unit 17, and the inlet-outlet unit 15, communicating with the operation unit 11.