A system for macroscopic and confocal imaging of tissue having a macroscopic imager for capturing a macroscopic image of the tissue's surface, a confocal imager for capturing one or more optically formed sectional microscopic images on or within tissue, a computer for receiving images from such imagers, and a tissue attachment device in which the macroscopic imager and confocal imager are each individually presented to the tissue utilizing the tissue attachment device in a predefined alignment, such that imaging locations of the confocal imager with respect to the tissue surface spatially correlate with macroscopic image. A user interface is operable on the computer to enable display of the macroscopic image on a display coupled to the computer, and to indicate a region within the macroscopic image associated with the field of view of the tissue imagable by the confocal imager. The user interface enables graphical tracking and targeting of imaging locations of the confocal imager in macroscopic image, and marking on the macroscopic image of the locations of confocal images with respect to the tissue surface.