An interactive security screening system includes a main housing, a contact pad provided on the main housing and a sample sheet positioned upon the contact pad. A sensor is operatively connected to the contact pad to measure pressure applied to the contact pad during a sample collection process. A feedback system, operatively connected to the contact pad and the sensor, provides the subject or screener with at least one of a visual signal and an audio signal to indicate whether requisite pressure was applied to the contact pad. A sample processing system analyzes the sample collection sheet through series, parallel or image processing to determine whether the trace residue sample contains a threat residue. The screening system also employs a spatial recognition system that focuses the sample processing system on particular portions of the sample collection sheet to increase overall system accuracy.