A separation fastener assembly including a base and a housing positioned adjacent the base. A nut is disposed in the cavity. A bolt extends through an aperture in the base and including an end coupled to the nut. A washer is positioned proximate the nut and operative to retain the nut within the housing. The washer includes a plurality of segments. An actuator is carried by the housing and is in communication with the cavity. The actuator is operative to displace the housing relative to the washer from a first position radially retaining plurality of segments of the washer and retaining the nut in the cavity to a second position permitting radial expansion of the plurality of segments and permitting withdrawal of the nut from the housing.