A manufacturing method of an optical film is provided. The method includes the following steps. A substrate is provided first and a surface of the substrate is aligned or an alignment layer is formed on the substrate. Next, a defined pattern layer is formed on the aligned surface of the substrate or on a surface of the alignment layer at a first temperature. A first liquid crystal layer is then coated on the aligned surface of the substrate or the surface of the alignment layer to cover the defined pattern layer. Then, a first curing step is performed at a second temperature to enable the defined pattern layer and the first liquid crystal layer to form the optical film. A portion of the optical film having the defined pattern layer is characterized by a different liquid crystal alignment property from the other portion of the optical film.