Invention Grant
US07709700B2 Method for improving plant transformation efficiency by adding copper ion 有权
通过添加铜离子提高植物转化效率的方法

  • Patent Title: Method for improving plant transformation efficiency by adding copper ion
  • Patent Title (中): 通过添加铜离子提高植物转化效率的方法
  • Application No.: US10567965
    Application Date: 2004-08-12
  • Publication No.: US07709700B2
    Publication Date: 2010-05-04
  • Inventor: Yuji Ishida
  • Applicant: Yuji Ishida
  • Applicant Address: JP Tokyo
  • Assignee: Japan Tobacco Inc.
  • Current Assignee: Japan Tobacco Inc.
  • Current Assignee Address: JP Tokyo
  • Agency: Birch, Stewart, Kolasch & Birch, LLP
  • Priority: JP2003-293062 20030813
  • International Application: PCT/JP2004/011599 WO 20040812
  • International Announcement: WO2005/017152 WO 20050224
  • Main IPC: C12N15/84
  • IPC: C12N15/84 A01H4/00
Method for improving plant transformation efficiency by adding copper ion
Abstract:
The present invention provides a method for Agrobacterium-mediated gene introduction into a plant material, comprising: 1) treating the plant material, and then 2) infecting the plant material with an Agrobacterium, characterized in that a medium enriched in a metal salt containing copper ion is used in step 1) and/or 2). The present invention also provides a process for preparing a transformed plant characterized in that the gene introduction method of the present invention is used.
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