Invention Grant
US07709608B2 Substrates useful for FRET protease assays for botulinum serotype A/E toxins
有权
用于肉毒杆菌血清型A / E毒素的FRET蛋白酶测定的底物
- Patent Title: Substrates useful for FRET protease assays for botulinum serotype A/E toxins
- Patent Title (中): 用于肉毒杆菌血清型A / E毒素的FRET蛋白酶测定的底物
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Application No.: US11609226Application Date: 2006-12-11
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Publication No.: US07709608B2Publication Date: 2010-05-04
- Inventor: Lance E. Steward , Ester Fernandez-Salas , Kei Roger Aoki
- Applicant: Lance E. Steward , Ester Fernandez-Salas , Kei Roger Aoki
- Applicant Address: US CA Irvine
- Assignee: Allergan, Inc.
- Current Assignee: Allergan, Inc.
- Current Assignee Address: US CA Irvine
- Agency: Allergan, Inc.
- Agent Dean G. Stathakis; Joel B. German
- Main IPC: C07K1/00
- IPC: C07K1/00 ; C07K2/00
Abstract:
The present invention provides clostridial toxin substrates useful in assaying for the protease activity of any clostridial toxin, including botulinum toxins of all serotypes as well as tetanus toxins. A clostridial toxin substrate of the invention contains a donor fluorophore; an acceptor having an absorbance spectrum overlapping the emission spectrum of the donor fluorophore; and a clostridial toxin recognition sequence that includes a cleavage site, where the cleavage site intervenes between the donor fluorophore and the acceptor and where, under the appropriate conditions, resonance energy transfer is exhibited between the donor fluorophore and the acceptor.
Public/Granted literature
- US20100069610A1 SUBSTRATES USEFUL FOR FRET PROTEASE ASSAYS FOR BOTULINUM SEROTYPE A/E TXOINS Public/Granted day:2010-03-18
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