A sterilization device for a stethoscope is provided, comprising a cover member and an ultraviolet light source. The stethoscope includes a chestpiece member having a face portion adapted to contact a patient. The cover member is adapted to operably engage the chestpiece member such that the cover member is movable with respect to the face portion and normally biased to cover the face portion. The ultraviolet light source is operably engaged with the cover member and configured to emit ultraviolet radiation for interacting with the face portion only when the cover member is covering the face portion. The ultraviolet light source thereby provides anti-microbial sterilization of at least the face portion of the chestpiece member. An associated apparatus is also provided.