Methods and systems for scan sequencing in an ultrasound imaging system are provided. The method includes determining an interleave factor based at least partially on a pulse repletion interval (PRI). The method further includes dividing a total number of colorflow imaging vectors into interleave groups based on the interleave factor. Further, the method includes modifying at least one scan sequencing parameter such that all colorflow imaging vectors are included in a colorflow region of interest (ROI).