An electro-wetting display panel including a first substrate, an insulator layer, a second substrate, partitioning structures, and electro-wetting display mediums. The first substrate has a plurality of first electrodes. The insulator layer is disposed on the first substrate to cover the first electrodes. The second substrate located above the first substrate and has a plurality of second electrodes. The partitioning structures are disposed on the insulator layer and each defines a pixel region, respectively. At least one of the partitioning structures has a flow channel surrounding the pixel regions, and the flow channel is connected to one of the pixel regions correspondingly. The electro-wetting display mediums are disposed within the pixel regions and the flow channels. When the electro-wetting display mediums are driven by the electric charge between the first electrodes and the second electrodes, the electro-wetting display mediums move between the pixel regions and the flow channels.