A microscope produces a high magnification stereoscopic image of a specimen by generating in the specimen two needle shaped illuminated regions tilted in optical axis of the microscope using a plurality of pinholes, moveable mirrors, and beamsplitters (37, 70). Light emitted from the needle shaped regions is detected using separate detectors (56, 57). The needle shaped regions are scanned with respect to the specimen using scanner (3). Alternately, a hologram may be used to generate the needle shaped illuminated regions.