A display substrate, a preparation method thereof and a display apparatus. The display substrate comprises a substrate, multiple pixel units arranged on the substrate and a color filter layer arranged on the pixel unit, wherein the color filter layer comprises color optical filters of different colors and a touch control structure layer arranged between the color optical filters of different colors; the touch control structure layer comprises a touch control connection electrode, a first coating protective layer covering the touch control connection electrode and a touch control electrode arranged on the first coating protective layer, the touch control electrodes comprise a first touch control electrode and a second touch control electrode, and at least one of the first touch control electrode and the second touch control electrode is connected with the touch control connection electrode through a via hole penetrating through the first coating protective layer.