A display substrate and a pixel driving circuit, the display substrate includes: a base substrate; a first conductive layer on the base substrate, the first conductive layer including a first signal line, a second signal line, and an additional pad layer spaced apart from each other; and a first electrode on a side of the first conductive layer away from the base substrate, where an orthographic projection of the first electrode on the base substrate and an orthographic projection of the additional pad layer on the base substrate have a second overlapping region, and the first conductive layer further includes a first electrical connection portion, and an orthographic projection of the first electrical connection portion on the base substrate does not overlap an orthographic projection of the second overlapping region on the base substrate.