A pixel circuit, a driving method, a display substrate, and a display device are provided. The pixel circuit includes a driving circuit, a first light-emitting control circuit, a light-emitting element, a first initialization circuit, and a second initialization circuit. The first initialization circuit writes, under the control of a first initialization control signal, an initialization voltage provided by an initialization voltage line, to a control terminal of the driving circuit; the second initialization circuit writes, under the control of a second initialization control signal provided by a second initialization control line, an initial data voltage provided by an initial data line, to an anode of the light-emitting element. Light emission of the light-emitting element from a leakage current and lateral leakage at low gray scales caused when initializing the anode of the light-emitting element can be prevented.