Aspects of the disclosure relate to real-time validation of application data. A computing platform may collect, in real-time, information associated with a plurality of data transmissions between applications, where the information includes, for each data transmission of the plurality of data transmissions, an indication of a source application and a destination application, a first indication whether the data transmission was sent by the source application, and a second indication whether the data transmission was received by the destination application. The computing platform may compare, for each data transmission, the first indication and the second indication. The computing platform may detect, for a particular data transmission, a lack of a match between the first indication and the second indication. The computing platform may identify the particular data transmission as an anomalous data transmission. Then, the computing platform may trigger one or more security actions to mitigate the anomalous data transmission.