- Patent Title: Tim protein-bound carrier, methods for obtaining, removing and detecting extracellular membrane vesicles and viruses using said carrier, and kit including said carrier
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Application No.: US17005225Application Date: 2020-08-27
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Publication No.: US11639924B2Publication Date: 2023-05-02
- Inventor: Takahiro Nishibu , Naoko Imawaka , Ken Naruse , Rikinari Hanayama
- Applicant: FUJIFILM WAKO PURE CHEMICAL CORPORATION , OSAKA UNIVERSITY
- Applicant Address: JP Osaka; JP Suita
- Assignee: FUJIFILM WAKO PURE CHEMICAL CORPORATION,OSAKA UNIVERSITY
- Current Assignee: FUJIFILM WAKO PURE CHEMICAL CORPORATION,OSAKA UNIVERSITY
- Current Assignee Address: JP Osaka; JP Suita
- Agency: Leydig, Voit & Mayer, Ltd.
- Priority: JPJP2014-246876 20141205
- Main IPC: G01N33/50
- IPC: G01N33/50 ; C07K1/14 ; C12N15/09 ; C07K17/00 ; C12N7/02 ; C07K14/705 ; C07K17/02 ; G01N33/543 ; G01N33/68 ; G01N33/53 ; G01N15/00 ; G01N15/10 ; G01N15/14
Abstract:
The invention provides a carrier and a method for obtaining, removing, or detecting extracellular membrane vesicle or virus present in a sample. In particular, the invention provides (a) a carrier (a Tim carrier) on which a protein (a Tim protein), selected from a T-cell immunoglobulin and mucin domain-containing molecule-4 (a Tim-4) protein, a Tim-3 protein, and a Tim-1 protein, is bound; (b) a method for obtaining the extracellular membrane vesicle or the virus in the sample; (c) a method for removing the extracellular membrane vesicle or the virus in the sample; (d) a method for detecting the extracellular membrane vesicle or the virus in the sample; (e) a kit for capturing the extracellular membrane vesicle or the virus, comprising the Tim carrier; and (f) a kit for capturing the extracellular membrane vesicle or the virus, comprising a reagent containing the Tim protein and a reagent containing the carrier.
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