Sounding Reference Signal (SRS) transmission for positioning can be utilized on flexible symbols. Techniques disclosed for transmitting a reference signal for positioning comprise receiving, from a serving base station, a message comprising an indication to transmit a SRS on a set of symbols of an orthogonal frequency division multiplexing (OFDM) slot. Techniques may also comprise determining the SRS is to be used for positioning, and receiving, from the serving base station, downlink control information (DCI) having a slot format indicator (SFI) that designates a subset of the set of symbols as flexible. Techniques may also comprise transmitting the SRS on at least a portion of the subset.