The embodiments provide a detection circuit and a detection method. The detection circuit includes an ESD protection device, a first fuse and a transistor. A first terminal of the ESD protection device is connected to a first terminal of the first fuse, and a connection terminal of the ESD protection device and the first fuse serves as a first test terminal; a second terminal of the first fuse is connected to a gate electrode of the transistor, and a connection terminal of the first fuse and the transistor serves as a second test terminal; and a second terminal of the ESD protection device is connected to at least one of a source electrode, drain electrode or substrate of the transistor, and a connection terminal of the ESD protection device and the transistor serves as a third test terminal.