Disclosed is an apparatus for separating and sorting USB wires. The apparatus comprises a separating apparatus for separating USB wires and a sorting apparatus provided downstream the separating apparatus. The separating apparatus comprises a first slider slidable in a horizontal plane in a reciprocating way; a second slider connected to the first slider via a sliding guide; and a teeth assembly comprising a group of first teeth and a group of second teeth. The sorting apparatus comprises a base plate; a wire holder fixed on the base plate and having a number of grooves for holding the wires therein; a sorting module arranged above the wire holder having a number of sorting slots corresponding to the number of the grooves; a gripping module mounted below the base plate and comprising a gripping head positioned at the interval between the wire holder and the sorting module; a vision module arranged above the interval for obtaining an image showing a location and a color of the wires in the grooves; and a control module for controlling the gripping head to grasp and move the wires to the corresponding slot.