Invention Grant
- Patent Title: Method for evaluating multiple different genes of interest
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Application No.: US16498618Application Date: 2018-03-30
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Publication No.: US11542497B2Publication Date: 2023-01-03
- Inventor: Hiroyuki Mano , Shinji Kohsaka , Masachika Ikegami
- Applicant: THE UNIVERSITY OF TOKYO
- Applicant Address: JP Tokyo
- Assignee: THE UNIVERSITY OF TOKYO
- Current Assignee: THE UNIVERSITY OF TOKYO
- Current Assignee Address: JP Tokyo
- Agency: Foley & Lardner LLP
- Priority: JPJP2017-069085 20170330
- International Application: PCT/JP2018/013539 WO 20180330
- International Announcement: WO2018/181863 WO 20181004
- Main IPC: C12N15/10
- IPC: C12N15/10 ; C12Q1/6886 ; G01N33/50 ; G01N33/574

Abstract:
It is an object of the present invention to provide, for instance, a method for evaluating a function, such as transforming potential, of multiple different genes of interest, and a method capable of evaluating drug sensitivity of a subject having each gene of interest. The present invention relates to, for instance, a method for evaluating multiple different genes of interest, comprising the steps of: integrating, into host cell genomic DNA, polynucleotides each comprising a tag sequence and a gene of interest or a fragment thereof linked to the tag sequence; mixing a plurality of different host cells having the different polynucleotides integrated therein; culturing the mixed host cells; extracting the genomic DNA from the cultured host cells; quantifying each of the polynucleotides in the extracted genomic DNA based on the tag sequence; and determining a relative cell count of each of the host cells having the respective polynucleotides after the culturing, based on the quantified values for the polynucleotides.
Public/Granted literature
- US20210189383A1 METHOD FOR EVALUATING MULTIPLE DIFFERENT GENES OF INTEREST Public/Granted day:2021-06-24
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