A display substrate and a method for driving the display substrate are provided in the present disclosure. The display substrate includes a plurality of pixel units arranged in an array and a power supply signal structure configured to apply a power supply voltage signal to each pixel unit. The power supply signal structure includes: a plurality of driving voltage signal VDD lines, a first power supply voltage signal VDD1 line connected to a first end of each driving voltage signal VDD line, and configured to apply a first power supply voltage V1 to the first end of the driving voltage signal line, and a second power supply voltage signal VDD2 line connected to a second end of the driving voltage signal VDD line, and configured to apply a power supply voltage V2 to the second end of the driving voltage signal VDD line.