A display panel and a method of manufacturing the display panel are provided. The display panel includes an array substrate, a pixel definition layer, and spacers. Each of spacers includes a bottom surface and a top surface. A cross-sectional area of the top surface is less than a cross-sectional area of the bottom surface. A horizontal distance from a center to a side of the spacer gradually increases from the top surface to the bottom surface. Moreover, holes of the mask plate corresponding to positions of the spacers are defined, which ensures accuracy of photolithography and display effect of the display panel.