The display substrate may include a base substrate (1), a display structure (20) on a display area (B) of the base substrate (1), at least one blocking structure on a peripheral area around the display area (B) of the base substrate (1), and a first water-blocking layer (10) on the substrate (1). The first water-blocking layer (10) may cover the display structure (20) and the at least one blocking structure. The blocking structure may be configured to block cracks generated on the first water-blocking layer (10) at a side of the blocking structure opposite from the display area (B) from propagating to the display area (B).