A naked-eye stereoscopic display device is provided herein, which includes: a display panel having a plurality of first pixel zones and second pixel zones, the first and second pixel zones are arranged alternately to be adjacent to one another and form an array; and a phase retardation film disposed on a surface of the display panel, the phase retardation film includes first and second retardation regions, each first retardation region has different value of phase retardation and is corresponding to one of the first pixel zones, and each second retardation region has different value of phase retardation and is corresponding to one of the second pixel zones. A naked-eye stereoscopic display system is also provided herein.