Aspects of the disclosure relate to real-time validation of data transmissions based on security profiles. A computing platform may collect, in real-time, information associated with a plurality of data transmissions between applications, where the information may include, for each data transmission, an indication of a source application and a destination application. Then, the computing platform may retrieve, from a repository and for each data transmission, a first security profile associated with the source application, and a second security profile associated with the destination application. The computing platform may then compare, for each data transmission, the first security profile to the second security profile. Subsequently, the computing platform may detect, based on a determination that the first security profile does not match the second security profile, a potentially unauthorized data transmission. Then, the computing platform may trigger one or more security actions to prevent the potentially unauthorized data transmission.