An electronic device may include a harmonic rejection mixer with a delay line, mixer array, and load. The delay line may generate LO phases. Each mixer in the array may have a first input that receives an LO phase and a second input coupled to an input switch and the first input of the next mixer circuit through an inter-mixer switch. The load may include a set of switches. In a transmit mode, the input switches and set of switches may be closed while the inter-mixer switches are open. In a self-calibration mode, the input switches and set of switches may be open while the inter-mixer switches are closed. A controller may sweep through phase codes for the programmable delay line while storing a digital output from the load. The controller may calibrate the phase code based on the digital output.