The present invention provides a test method and a device of data mischarging of a display panel. The test method includes: a personal computer (PC) controlling a pixel group to output a screen with a solid color; controlling a charging time of a master device to be constant and adjusting a charging time of a timing controller; determining whether a measurement data of an optical test instrument is greater than a first threshold and detecting whether an obvious boundary appears in a middle of the display panel, and determining a mischarging time if so.