Disclosed is an automatic analysis device (100), including: a dispensing unit (20), configured to dispense a sample and/or a reagent into a reaction vessel; a reaction unit (10), configured to incubate the reactant in the reaction vessel and transfer the reaction vessel; a B/F unit (2), configured to wash the reactant in the reaction vessel; a detecting unit (1), configured to detect a reaction signal in the reaction vessel; wherein the reaction unit (10) includes one rotating device (11) provided with a reaction vessel position to bear and fix the reaction vessel. The technical solution of the disclosure eliminates the washing tray and the detecting tray, simplifies the system structure and the control flow, and also significantly reduces the size of the reaction unit (10), implements flexible incubation time, and improves the working efficiency of the analysis device (100).