A microfluidic valve provided herein is configured to mix or capable of mixing a sample and/or a reagent in addition to controlling liquid flow. In one embodiment, the microfluidic valve comprises a rotor (3) and one or more micro-structures (2) that move with the rotation of the rotor (3). In one embodiment, the one or more micro-structures (2) stir and/or mix content in a mixing chamber (5) formed by the rotor (3), a base (1), and a sleeve (4) of the microfluidic valve. A microfluidic chip or chip system comprising one or more of the microfluidic valves, and methods of use, are also provided.