- Patent Title: Methods for high fidelity production of long nucleic acid molecules
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Application No.: US15359560Application Date: 2016-11-22
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Publication No.: US11268115B2Publication Date: 2022-03-08
- Inventor: Peter A. Carr , Brian Y. Chow , Joseph M. Jacobson , David W. Mosley , Christopher Emig
- Applicant: Massachusetts Institute of Technology
- Applicant Address: US MA Cambridge
- Assignee: Massachusetts Institute of Technology
- Current Assignee: Massachusetts Institute of Technology
- Current Assignee Address: US MA Cambridge
- Agent Norma E. Henderson
- Main IPC: C12Q1/68
- IPC: C12Q1/68 ; C12P19/34 ; C12Q1/6848 ; C12Q1/6825 ; C12Q1/6834

Abstract:
In a method for generating an elongated nucleic acid molecule, a nucleic acid addition of a first nucleic acid molecule attached to a first 3′ or 5′ protecting group to a nucleic acid immobilized on a surface produces an intermediate-length immobilized nucleic acid. The first protecting group is dissociated from the first nucleic acid molecule. A second nucleic acid molecule that is attached to a second associated a 3′ or 5′ associated protecting group is added to the intermediate-length nucleic acid. The second associated protecting group is dissociated from the second nucleic acid molecule. A sequentially-extended elongated immobilized nucleic acid molecule having a desired sequence and length is produced by sequentially extending the intermediate-length immobilized nucleic acid by adding additional nucleic acid molecules with associated protecting groups to the intermediate-length nucleic acid and dissociating the associated protecting group after each addition.
Public/Granted literature
- US20170137858A1 Methods for High Fidelity Production of Long Nucleic Acid Molecules Public/Granted day:2017-05-18
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