A display device and a driving protection method thereof are provided. The display device includes a timing controller and a source driver. The timing controller encrypts a verification data to generate a first encryption signal. The source driver coupled to the timing controller receives the first encryption signal. The source driver decrypts the first encryption signal to obtain a first decryption data. The source driver encrypts the first decryption data to generate a second encryption signal. The source driver outputs the second encryption signal to the timing controller. The timing controller decrypts the second encryption signal to obtain a second decryption data. When the timing controller determines that the second decryption data matches the verification data, the timing controller enables the source driver to perform display driving.