The present disclosure relates to the field of display technologies, and relates to a display substrate, a fabricating method of a display substrate, and a display device. The display substrate includes a base. The display substrate is divided into a display area and a non-display area located at a periphery of the display area. The display area has a plurality of pixel regions. Each of pixel regions is provided with a pixel structure. The pixel structure includes a plurality of organic film layers and inorganic film layers disposed in a stacked manner. An area of the non-display area near an edge thereof is an anti-cracking reinforcing area, which is only provided with the organic film layer. The organic film layer at least covers an outer edge surface of the inorganic film layer adjacent to the anti-cracking reinforcing area in the non-display area.