A light-emitting control circuit and a driving method of a display device are disclosed. The display device includes a plurality of rows of sub-pixels; each row of sub-pixels includes at least first color sub-pixels and second color sub-pixels; each of the first and second color sub-pixels includes a pixel circuit and a light-emitting unit; the light-emitting control circuit includes a plurality of light-emitting control circuit groups which are in a one-to-one correspondence with the plurality of rows of sub-pixels; each of the plurality of light-emitting control circuit groups at least includes a first light-emitting control sub-circuit and a second light-emitting control sub-circuit; the first and second light-emitting control sub-circuit provide a first light-emitting control signal and a second light-emitting control signal, respectively; and each of the first and second light-emitting control signal is used for driving the pixel circuit to output a driving current to the light-emitting unit.